HI83303 is a compact, multiparameter photometer for use in aquaculture applications. The meter is one of the most advanced photometers available with an innovative optical design that utilizes a reference detector and focusing lens to eliminate errors from changes in the light source and from imperfections in the glass cuvette. This meter has 20 different programmed methods measuring 12 key water quality parameters and also offers an absorbance measurement mode for performance verification and for users that would like to develop their own concentration versus absorbance curves. Aquaculture parameters include alkalinity, calcium, nitrite, and phosphate which are critical to maintaining a healthy system. It also contains parameters specific to either a marine or freshwater environment.
To save valuable laboratory benchtop space, the HI83303 doubles as a professional pH meter with its digital pH/temperature electrode input. Now one meter can be used for both photometric and pH measurements.
- Advanced optical system
- Digital pH electrode input
- Absorbance Measuring Mode
The HI83303 benchtop photometer measures 12 different key water quality parameters using 20 different methods. This photometer features an innovative optical system that uses LEDs, narrow band interference filters, focusing lens and both a silicon photodetector for absorbance measurement and a reference detector to maintain a consistent light source ensures accurate and repeatable photometric readings every time.
Made with the aquaculture industry in mind, the HI83303 is a comprehensive solution to maintaining optimal chemical and environmental conditions, preventing disease and increasing production. The HI83303 measures vital parameters such as alkalinity, calcium, nitrite, and phosphate. Alkalinity plays a part in a dynamic relationship with pH and CO2 concentrations, high alkalinity water lowers fluctuations in pH. The buffering capacity acts to store extra CO2 essential for photosynthesis in the ponds to produce oxygen. Maintaining calcium at certain levels is vital to proper fish growth and development. Excessive nitrite can be toxic to fish. When nitrite interacts with hemoglobin the iron becomes oxidized and the blood cell can no longer carry oxygen. Phosphate is essential to plant growth; too much phosphate in an aquaculture system can contribute to algal blooms decreasing dissolved oxygen vital for a successful ecosystem.
A digital pH electrode input allows the user to measure pH by a traditional glass electrode. The digital pH electrode has a built-in microchip within the probe that stores all of the calibration information. Having the calibration information stored in the probe allows for hot swapping of pH electrodes without having to recalibrate. All pH measurements are automatically compensated for temperature variations with a built-in thermistor located in the tip of the sensing bulb for fast and accurate temperature measurement.
The HI83303 offers an absorbance measuring mode that allows for CAL Check standards to be used to validate the performance of the system. The absorbance mode allows the user to select one of the five wavelengths of light (420 nm, 466 nm, 525 nm, 575 nm, and 610 nm) to measure and plot their own concentration versus absorbance mode. This is useful for users with their own chemical method and for educators to teach the concept of absorbance by using the Beer-Lambert Law.
Two USB ports are provided for transferring data to a flash drive or computer and to use as a power source for the meter. For added convenience and portability the meter can also operate on an internal 3.7 VDC Lithium-polymer rechargeable battery.
Backlit 128 x 64 Pixel Graphic LCD Display
- Backlit graphic display allows for easy viewing in low light conditions
- The 128 x 64 Pixel LCD allows for a simplified user interface with virtual keys and on-screen help to guide the user through use of the meter
Built-in Reaction Timer for Photometric Measurements
- The measurement is taken after the countdown timer expires.
- Countdown timer ensures that all readings are taken at the appropriate reaction intervals regardless of user for better consistency in measurements
- Hanna's exclusive CAL Check cuvettes for validation of light source and detector
- Allows for the user to plot concentration versus absorbance for a specific wavelength for use with user-supplied chemistry or for teaching principles of photometry
Units of Measure
- Appropriate unit of measure along with chemical form is displayed along with reading
- Automatically convert readings to other chemical forms with the touch of a button
- Aids in preventing stray light from affecting measurements
Digital pH Electrode Input
- Measure pH and temperature with a single probe
- Good Laboratory Practice (GLP) to track calibration information including date, time, buffers used, offset and slope for traceability
- pH CAL Check alerts user to potential problems during the calibration process
- Space saving having a pH meter and photometer built into one meter
- Up to 1000 photometric and pH readings can be stored by simply pressing the dedicated LOG button. Logged readings are just as easily recalled by pressing the RCL button
- Sample ID and User ID information can be added to a logged reading using alphanumeric keypad
- Logged readings can be quickly and easily transferred to a flash drive using the USB-A host port or to a computer using the micro USB-B port
- Data is exported as a .CSV file for use with common spreadsheet programs
Battery Status Indicator
- Indicates the amount of battery life left
- Photometric error messages include no cap, high zero, and standard too low
- pH calibration messages include clean electrode, check buffer and check probe
Advanced Optical System
HI83303 is designed with an innovative optical system that incorporates a beam splitter so that light can be used for absorbance readings and for a reference detector. The reference detector monitors the intensity of light and modulates when there is drift due to power fluctuation or the heating of the optical components. Each part has an important role in providing unparalleled performance from a photometer.
High Efficiency LED Light Source
A LED light source offers superior performance as compared to a tungsten lamp. LEDs have a much higher luminous efficiency, providing more light while using less power. They also produce very little heat, which could otherwise affect the optical components an electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps are supposed to be white light (all wavelengths of visible light) but actually have a poor blue/violet light output.
High-Quality Narrow Band Interference Filters
The narrow band interference filter not only ensure greater wavelength accuracy (+/- 1 nm) but are extremely efficient. The filters used allow up to 95% of the light from the LED to be transmitted as compared to other filters that are only 75% efficient. The higher efficiency allows for a brighter, stronger light source. The end result is higher measurement stability and less wavelength error.
Reference Detector for a Stable Light Source
A beam splitter is used as part of the internal reference system of the HI83303 photometer. The reference detector compensates for any drift due to power fluctuations or ambient temperature changes. Now you can rely on a stable source of light between your blank (zero) measurement and sample measurement.
Large Cuvette Size
The sample cell of the HI83303 fits a round, glass cuvette with a 25 mm path length. Along with the advanced optical components, the larger size of the cuvette greatly reduces errors in rotation from the indexing mark of the cuvettes. The relatively long path length of the sample cuvette allows the light to pass through more of the sample solution, ensuring accurate measurements even in low absorbance samples.
Focusing Lens for Greater Light Yield
Adding a focusing lens to the optical path allows for the collection of all of the light that exits the cuvette and focusing the light on the silicon photo detector. This novel approach to photometric measurements cancels the errors from imperfections and scratches present in the glass cuvette eliminating the need to index the cuvette.
Accuracy(at 25 °C)
|Absorbance||0.000 to 4.000 Abs||0.001 Abs||+/-0.003Abs @ 1.000 Abs|
|Alkalinity||0 to 500 mg/L (as CaCO3 )||1 mg/L||±5 mg/L ±5% of reading||Colorimetric Method||610 nm||HI775-26|
|Alkalinity, Marine||0 to 300 mg/L (as CaCO3 )||1 mg/L||±5 mg/L ±5% of reading||Colorimetric Method||610 nm||HI755-26|
|Ammonia, Low Range||0.00 to 3.00 mg/L (as NH3 -N)||0.01 mg/L||±0.04 mg/L ±4% of reading||Adaptation of the ASTM Manual of Water and Environmental Technology, D1426 Nessler method.||420 nm||HI93700-01|
|Ammonia, Medium Range||0.00 to10.00 mg/L (as NH3 -N)||0.01 mg/L||±0.05 mg/L ±5% of reading||Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method||420 nm||HI93715-01|
|Ammonia, High Range||0.0 to 100.0 mg/L (as NH3 -N)||0.1 mg/L||±0.5 mg/L ±5% of reading||Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method||420 nm||HI93733-01|
|Calcium||0 to 400 mg/L (as Ca2+)||1 mg/L||±10 mg/L ±5% of reading||Adaptation of the Oxalate method||466nm||HI937521-01|
|Calcium, Marine||200 to 600 mg/L (as Ca2+)||1 mg/L||±6% of reading||Adaptation of the Zincon method||610 nm||HI758-26|
|Chlorine, Free||0.00 to 5.00 mg/L (as Cl2 )||0.01 mg/L||±0.03 mg/L ±3% of reading||Adaptation of the EPA DPD method 330.5||525 nm||HI93701-01|
|Chlorine, Total||0.00 to 5.00 mg/L (as Cl2 )||0.01 mg/L||±0.03 mg/L ±3% of reading||Adaptation of the EPA DPD method 330.5||525 nm||HI93711-01|
|Copper, Low Range||0.000 to 1.500 mg/L (as Cu)||0.001 mg/L||±0.010 mg/L ±5% of reading||Adaptation of the EPA method||575 nm||HI95747-01|
|Copper, High Range||0.00 to 5.00 mg/L (as Cu)||0.01 mg/L||±0.02 mg/L ±4% of reading||Adaptation of the EPA method||575 nm||HI93702-01|
|Nitrate||0.0 to 30.0 mg/L (as NO3 - N)||0.1 mg/L||±0.5 mg/L ±10% of reading||Adaptation of the cadmium reduction method||525 nm||HI93728-01|
|Nitrite, Marine Ultra Low Range||0 to 200 µg/L (as N02-N)||1 µg/L||±10 µg/L ±4% of reading||Adaptation of the EPA Diazotization method 354.1||466 nm||HI764-25|
|Nitrite, Low Range||0 to 600 µg/L (as NO2-N)||1 µg/L||±20 µg/L ±4% of reading||Adaptation of the EPA Diazotization method 354.1||466 nm||HI93707-01|
|Nitrite, High Range||0 to 150 mg/L (as NO2-)||1 mg/L||±4 mg/L ±4% of reading||Adaptation of the Ferrous Sulfate method||575 nm||HI93708-01|
|Oxygen, Dissolved||0.0 to 10.0 mg/L (as O2 )||0.1 mg/L||±0.4 mg/L ±3% of reading||Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Azide modified Winkler method||420 nm||HI93732-01|
|pH||6.5 to 8.5 pH||0.1 pH||±0.1 pH||Adaptation of the Phenol Red method||525 nm||HI93710-01|
|Phosphate, Marine Ultr Low Range||0 to 200 µg/L (as P)||1 µg/L||±5 µg/L ±5% of reading||Adaptation of the Standard Methods for the Examination of Water and Wastewater, 20th edition, Ascorbic Acid method||610 nm||HI736-25|
|Phosphate, Low Range||0.00 to 2.50 mg/L (as PO4 3-)||0.01 mg/L||±0.04 mg/L ±4% of reading||Adaptation of the Ascorbic Acid method||610 nm||HI93713-01|
|Phosphate, High Range||0.0 to 30.0 mg/L (as PO4 3-)||0.1 mg/L||±1.0 mg/L ±4% of reading||Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method||525 nm||HI93717-01|
|Wavelength Accuracy||±1 nm|
|Light Source||5 LEDs with 420 nm, 466 nm, 525 nm, 575 nm, and 610 nm narrow band interference filters|
|Spectral bandwidth||8 nm|
|Input Channels||1 pH electrode input and 5 photometer wavelengths|
|pH Electrode||digital pH electrode (not included)|
|Logging Type||log on demand with user name and sample ID optional input|
|Logging Memory||1000 readings|
|Connectivity||USB-A host for flash drive; micro-USB-B for power and computer connectivity|
|GLP||calibration data for connected pH electrode|
|Display||128 x 64 pixel LCD with backlight|
|Battery Type / Life||3.7 VDC Li-polymer rechargeable battery / >500 photometric measurements or 50 hours of continuous pH measurement|
|Power Supply||5 VDC USB 2.0 power adapter with USB-A to micro-USB-B cable (included)|
|Environment||0 to 50.0 oC (32 to 122.0 oF); 0 to 95% RH, non-condensing|
|Dimensions||206 x 177 x 97 mm (8.1 x 7.0 x 3.8")|
|Weight||1.0 kg (2.2 lbs.)|
|Photometer/Colorimeter Light Detector||silicon photodetector|
|Cuvette Type||round, 24.6 mm|
|Number of Methods||128 max.|
|Ordering Information||HI83303 is supplied with sample cuvettes and caps (4 ea.), cloth for wiping cuvettes, USB to micro USB cable connector, power adapter and instruction manual.
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